Cytotoxic potential on human breast and lung cancer cells of the biosynthesized gold nanoparticles from the reduction of chloroauric acid by lactic acid isolated from Lactobacillus acidophilus

Chemotherapy side effects, drug resistance, and tumor metastasis hinder the progress of cancer treatments, which has led to patients having a poor prognosis. In recent years, nanoparticles (NPs) have become an emerging trend in delivery technology. The biosynthesis gold (AuNPs) from lactic acid as probiotic metabolites is cost-efficient eco-friendly approach due widespread availability ease culturing methods. Here, AuNPs were synthesized by reducing chloroauric using isolated Lactobacillus acidophilus . Surface characterization biosynthesized was performed UV-visible spectroscopy, field emission scanning electron microscopy with energy dispersive spectroscopy (FESEM-EDS), atomic force (AFM). distinct peak UV-Vis spectra at around 520 nm indicates nanoscale level particles. SEM images shape found be small, smooth spherical particles sizes ranging 6 12 nm. AFM results agree images, showing 4 15 anticancer activities been investigated cytotoxicity (MTT) apoptosis (Caspase 3/7) assay human breast lung cells (MCF7 A549), control (myoblasts). MTT showed AuNPs’ cytotoxic potential on A549) compared normal Cytotoxicity exhibit toxicity against adenocarcinoma (MCF7, IC 50 0.075 mM) (A549, 0.07 mM), shown evidently cellular morphology DNA-stained nucleus. Only MCF7 exhibited apoptotic events upon AuNP treatment. proved safe they are non-toxic myoblasts. Interestingly, absorbed present cytosol, thus demonstrating selectivity toward used. study first evidence that can agent capping suggested chemotherapeutic leads targeted cancers.